Bioactivity Assays of <i>Eryngium thyrsoideum</i>; Focusing on Cytotoxic Effects, Antioxidant Activity, and Antimalarial Properties

Background: The genus Eryngium, Apiaceae is widely distributed in the world and used traditional medicine for different therapeutic purposes. Eryngium thyrsoideum was investigated due to a lack of phytochemical studies on probable bioactivities its compounds. Methods: aerial parts plant were extracted using n-hexane, dichloromethane, methanol (MeOH) by soxhlet method. MeOH extract exposed C18 Sep-Pak fractionation step gradient MeOH-H2 O. essential oil obtained Clevenger apparatus. Free-radical scavenging activity extracts fractions assessed DPPH-scavenging Total flavonoid content determined Aluminum chloride colorimetric phenolic Folin-Ciocalteu reagent α-Glucosidase inhibitory antimalarial cytotoxic oils also established this study. Results: 20% solid phase extraction fraction demonstrated high antioxidant (0.13±0.01 mg/mL) indicated most total contents (474.20±23.11 27.90±0.55 mg/100 g) respectively, comparison other fractions. We observed moderate β-Hematin Formation Assay effect (10.37±0.02 DCM (7.64±0.01 mg/mL). Low levels α-glucosidase inhibition n-hexane (9.78±1.1%) (2.7±1.1%). Cytotoxic confirmed representing respectively IC50 with 2.51 mcg/mL 1.07 24 48 hours MCF-7 cell lines. Conclusion: presence compounds flavonoids effective potency E. thyrsoideum. Essential presented noticeable effects human breast cancer